Promega 5x passive lysis buffer
WebCells were harvested 16 hours after transfection and lysed in the passive lysis buffer (Promega). Luciferase and Renilla activities were measured with a luminometer ... galactopyranoside (ONPG) as a substrate. Cells were lysed either in the passive lysis buffer or in the TBS lysis buffer (20 mM Tris, pH7.5, 150 mM NaCl and 0.5% NP-40) for IRF3 ... Web30ml. Passive Lysis Buffer, 5X, is specifically formulated to promote rapid lysis of cultured mammalian cells without the need for scraping adherent cells or performing freeze-thaw …
Promega 5x passive lysis buffer
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WebNov 6, 2006 · The Dual-Luciferase® Reporter (DLR™) Assay System is widely used for performing two reporter assays. Each system includes 10 ml Luciferase Assay Buffer II, 1 vial of Luciferase Assay Substrate, 10 ml of Stop & Glo Buffer, 200 ul of Stop and Glo Substrate (50X) and 30 ml of Passive Lysis Buffer (5X). A single sample can be used... WebPassive Lysis 5X Buffer 30ml ₩ 149,000 Your price: Log in Passive Lysis Buffer, 5X, is specifically formulated to promote rapid lysis of cultured mammalian cells without the …
WebThe Dual-Luciferase Reporter (DLR) Assay System provides an efficient means of performing two reporter assays. In the DLR Assay, the activities of firefly and Renilla luciferases are measured sequentially from a single sample. Includes: Luciferase Assay Buffer II; Luciferase Assay Substrate (lyophilized product); Stop & Glo Buffer; Passive ... WebLysis: For lysis of adherent cells, we recommend the following: (all reagents and lysates must be kept cold) 1. Treat cells as desired. 2. Wash plate with PBS to remove residual media. 3. Add 400 µL of 1x lysis buffer/ 10 cm …
Web30ml. ¥ 7,500. Luciferase Cell Culture Lysis 5X Reagent is used with the Luciferase Assay System (Cat.#. E1500), developed for reporter quantitation in mammalian cells. This lysis buffer provides rapid lysis of a variety of cell types, including adherent and nonadherent mammalian cells, bacterial cells, plant cells and tissue homogenates. WebIf additional lysis buffer is required for Renilla Luciferase Assay Kit 2.0 ( 30082 ), purchase 5X Passive Lysis Buffer ( 99912 ). More Luciferase Kits You may also like… ATP-Glo™ Bioluminometric Cell Viability Assay ATP …
WebApr 6, 2024 · The key global manufacturers of Luciferase Assay Lysis Buffer include Promega, Thermo Fisher Scientific, OriGene, GoldBio, Biotium, Atlas Biyoteknoloji, G Biosciences and New England Biolabs, etc ...
WebPassive Lysis Buffer, 5X. E1941 PDF (200 KB) – English (United States) suffolk university dean of studentsWeb86. Buy from Supplier. Promega. 5x Renilla Luciferase Assay Lysis Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … suffolk university data breachWeb1 5X Passive Lysis Buffer (Promega). Store at ‐20°C. 1x solution in ddH2O can be stored at 4°C for up to one month. ... 3 Add 100µl of 1x Passive lysis buffer and shake at room temperature for 10 min. 4 Transfere the lysate to a 1.5‐ml microcentrifuge tube and spin down the cells at 13000 rpm at room ... suffolk university employment opportunitiesWebPromega's Cookie Policy Close. We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and … suffolk university human resources jobsWebThis lysis buffer is strong enough for lysis most cell lines and wild enough to keep protein interactions. It has been tested on mouse embryonic stem cells, cancer cell lines and... paint removal tools for tight spacesWeb(E1941) Passive Lysis 5X Buffer - Promega - CiteAb 2024 CiteAb Awards - View the winners now! (E1941) Passive Lysis 5X Buffer Sign in or register to save this reagent to your favourites Supplier Promega View Product On Supplier's Website Supplier provided information Applications None provided Experiments from the literature Applications ? paint removal tools for drillspaint removal tools home depot